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plasmids pegfp c2 sept9 i1  (Addgene inc)


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    Structured Review

    Addgene inc plasmids pegfp c2 sept9 i1
    Plasmids Pegfp C2 Sept9 I1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+pegfp+c2+sept9+i1/pEGFP-C2_SEPT9+i1+(Plasmid+%2371609)/bio_rxiv__64898__2026__01__21__700789-222-6-8
    Average 92 stars, based on 3 article reviews
    plasmids pegfp c2 sept9 i1 - by Bioz Stars, 2026-09
    92/100 stars

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    Transfection:

    Article Title: Septins buffer actomyosin forces to protect the nucleus from genotoxic mechanical stress
    Article Snippet: Live imaging of cells during confined migration through the pores of Transwell membranes was performed by cutting a rectangle strip from the Transwell’s membrane insert and immobilizing it on the glass portion of a MatTek 35 mm glass bottom dish using a 4-well insert with an adhesive biocompatible bottom surface (ibidi, 80466/80469), which was placed on top of the filter and filled with HyClone DMEM/High Modified media. .. Cells were transfected transiently with the plasmids pEGFP-C2_SEPT9_i1 (addgene plasmid #71609) or LAP2 Full I pAcGFP-N1 monomeric GFP (addgene plasmid #62044; gift from Dr. Eric Schirmer) using Lipofectamine 2000 transfection reagent (Invitrogen, 11668019) according to the manufacturer’s instructions with minor optimization. .. Transfections were performed on 35 mm dishes using Opti-MEMTM I Reduced Serum Medium (Gibco, 31985070).

    Plasmid Preparation:

    Article Title: Septins buffer actomyosin forces to protect the nucleus from genotoxic mechanical stress
    Article Snippet: Live imaging of cells during confined migration through the pores of Transwell membranes was performed by cutting a rectangle strip from the Transwell’s membrane insert and immobilizing it on the glass portion of a MatTek 35 mm glass bottom dish using a 4-well insert with an adhesive biocompatible bottom surface (ibidi, 80466/80469), which was placed on top of the filter and filled with HyClone DMEM/High Modified media. .. Cells were transfected transiently with the plasmids pEGFP-C2_SEPT9_i1 (addgene plasmid #71609) or LAP2 Full I pAcGFP-N1 monomeric GFP (addgene plasmid #62044; gift from Dr. Eric Schirmer) using Lipofectamine 2000 transfection reagent (Invitrogen, 11668019) according to the manufacturer’s instructions with minor optimization. .. Transfections were performed on 35 mm dishes using Opti-MEMTM I Reduced Serum Medium (Gibco, 31985070).



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    plasmids pegfp c2 sept9 i1  (Addgene inc)
    92
    Addgene inc plasmids pegfp c2 sept9 i1
    Plasmids Pegfp C2 Sept9 I1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+pegfp+c2+sept9+i1/pEGFP-C2_SEPT9+i1+(Plasmid+%2371609)/bio_rxiv__64898__2026__01__21__700789-222-6-8
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    plasmids pegfp c2 sept9 i1 - by Bioz Stars, 2026-09
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    pegfp c2 sept9 i1  (Addgene inc)
    92
    Addgene inc pegfp c2 sept9 i1
    Pegfp C2 Sept9 I1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+pegfp+c2+sept9+i1/pEGFP-C2_SEPT9+i1+(Plasmid+%2371609)/pm38170778-192-7-9
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